Composition of the Vaginal Microbiota in Women of Reproductive Age – Sensitive and Specific Molecular Diagnosis of Bacterial Vaginosis Is Possible?

Composition of the Vaginal Microbiota in Women of Reproductive Age – Sensitive and Specific Molecular Diagnosis of Bacterial Vaginosis Is Possible? Научная публикация

Журнал

PLOS ONE
ISSN: 1932-6203

Вых. Данные

Год: 2013, Том: 8, Номер: 4, Номер статьи : e60670, Страниц : DOI: 10.1371/journal.pone.0060670

Авторы

Shipitsyna Elena ¹ , Roos Annika ² , Datcu Raluca ³ , Hallén Anders ⁴ , Fredlund Hans ⁵ , Jensen Jørgen S. ³ , Engstrand Lars ² , Unemo Magnus ⁵

Организации

1	Laboratory of Microbiology, D. O. Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia
2	Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Solna, Sweden
3	Department of Microbiological Surveillance and Research, Statens Serum Institut, Copenhagen, Denmark
4	Department of Dermatology and Venereology, Uppsala University Hospital, Uppsala, Sweden
5	World Health Organization Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Örebro University Hospital, Örebro, Sweden

Background and Objective: Bacterial vaginosis (BV) is the most common vaginal disorder, characterized by depletion of the normal lactobacillus-dominant microbiota and overgrowth of commensal anaerobic bacteria. This study aimed to investigate the composition of the vaginal microbiota in women of reproductive age (healthy women and women with BV), with the view of developing molecular criteria for BV diagnosis. Materials and Methods: Vaginal samples from 163 women (79 control, 73 BV and 11 intermediate (Lactobacillary grade II flora) cases) were analyzed using 454 pyrosequencing of the hypervariable regions V3-V4 of the 16S rRNA gene and 16 quantitative bacterial species/genus-specific real-time PCR assays. Sensitivities and specificities of potential BV markers were computed using the Amsel criteria as reference standard for BV. The use of quantitative thresholds for prediction of BV, determined for both relative abundance measured with 454 pyrosequencing and bacterial load measured with qPCR, was evaluated. Results: Relative to the healthy women, the BV patients had in their vaginal microbiota significantly higher prevalence, loads and relative abundances of the majority of BV associated bacteria. However, only Gardnerella vaginalis, Atopobium vaginae, Eggerthella, Prevotella, BVAB2 and Megasphaera type 1 detected at or above optimal thresholds were highly predictable for BV, with the best diagnostic accuracy shown for A. vaginae. The depletion of Lactobacillus species combined with the presence of either G. vaginalis or A. vaginae at diagnostic levels was a highly accurate BV predictor. Conclusions: Quantitative determination of the presence of G. vaginalis, A. vaginae, Eggerthella, Prevotella, BVAB2 and Megasphaera type 1 as well as the depletion of Lactobacillus was highly accurate for BV diagnosis. Measurements of abundance of normal and BV microbiota relative to total bacteria in vaginal fluid may provide more accurate BV diagnosis, and be used for test-of-cure, rather than qualitative detection or absolute counts of BV related microorganisms.

Shipitsyna E. , Roos A. , Datcu R. , Hallén A. , Fredlund H. , Jensen J.S. , Engstrand L. , Unemo M.
Composition of the Vaginal Microbiota in Women of Reproductive Age – Sensitive and Specific Molecular Diagnosis of Bacterial Vaginosis Is Possible?
PLOS ONE. 2013. V.8. N4. e60670 . DOI: 10.1371/journal.pone.0060670 WOS Scopus РИНЦ OpenAlex

Поступила в редакцию:	19 нояб. 2012 г.
Принята к публикации:	1 мар. 2013 г.
Опубликована online:	9 апр. 2013 г.

Web of science:	WOS:000317909600034
Scopus:	eid_2-s2.0-84876049369
РИНЦ:	20431093
OpenAlex:	W2062055186

БД	Цитирований
OpenAlex	218
Web of science	164