Pooling Samples: the Key to Sensitive, Specific and Cost-effective Genetic Diagnosis of Chlamydia trachomatis in Low-Resource Countries

Pooling Samples: the Key to Sensitive, Specific and Cost-effective Genetic Diagnosis of Chlamydia trachomatis in Low-Resource Countries Full article

Journal

Acta Dermato-Venereologica
ISSN: 0001-5555

Output data

Year: 2007, Volume: 87, Number: 2, Pages: 140-143 Pages count : 4 DOI: 10.2340/00015555-0196

Authors

Shipitsyna Elena ¹ , Shalepo Kira ¹ , Savicheva Alevtina ¹ , Unemo Magnus ² , Domeika Marius ³

Affiliations

1	Laboratory of Microbiology, DO Ott Research Institute of Obstetrics and Gynecology RAMS, St Petersburg, Russia
2	Department of Clinical Microbiology, Örebro University Hospital, Örebro, Sweden
3	Department of Medical Sciences, Uppsala University, Uppsala

The aims of this study were to compare the performance characteristics and cost-effectiveness of pooling endocervical samples for screening and diagnosis of Chlamydia trachomatis, and to investigate the prevalence of C. trachomatis infection in women in Leningrad Oblast, Russia. A total of 1500 endocervical samples were tested individually and when pooled in groups of 5 and 10 samples, respectively. A previously evaluated in-house diagnostic polymerase chain reaction (PCR) assay was utilized. The sensitivity and specificity of the PCR were not affected by either pooling strategy. The estimated prevalence of genital C. trachomatis infection was 6.6%, 6.1% and 6.0% based on individually tested samples, and pools of 5 and 10, respectively. For diagnosis of individual samples, the pooling strategies resulted in cost savings of 53.3% (5 samples per pool) and 44.0% (10 samples per pool). Pooling samples for PCR detection of C. trachomatis is an accurate and cost-saving approach for diagnosis and large-scale prevalence studies in St Petersburg, Russia.

Shipitsyna E. , Shalepo K. , Savicheva A. , Unemo M. , Domeika M.
Pooling Samples: the Key to Sensitive, Specific and Cost-effective Genetic Diagnosis of Chlamydia trachomatis in Low-Resource Countries
Acta Dermato-Venereologica. 2007. V.87. N2. P.140-143. DOI: 10.2340/00015555-0196 WOS Scopus РИНЦ OpenAlex

Published online:

Sep 4, 2006

Web of science:	WOS:000245590700006
Scopus:	eid_2-s2.0-34047108634
Elibrary:	13548560
OpenAlex:	W2115662011

DB	Citing
OpenAlex	38
Web of science	29