Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19 Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2022, Volume: 23, Number: 17, Article number : 10060, Pages count : DOI: 10.3390/ijms231710060

Tags

8-hydroxy-2′-deoxyguanosine; COVID-19; DNA fragmentation; catalase; nitrotyrosine; sperm; superoxide dismutase; total antioxidant capacity; uric acid; zinc.

Authors

Shcherbitskaia Anastasiia D. ¹ , Komarova Evgeniia M. ¹ , Milyutina Yulia P. ¹ , Ishchuk Mariia A. ¹ , Sagurova Yanina M. ¹ , Safaryan Galina K. ¹ , Lesik Elena A. ¹ , Gzgzyan Alexander M. ¹ , Bespalova Olesya N. ¹ , Kogan Igor Y. ¹

Affiliations

1	D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductive Medicine, 199034 St. Petersburg, Russia

SARS-CoV-2 negatively affects semen characteristics, impairs various biochemical processes in seminal fluid and within spermatogenic cells ultimately leading to male fertility decline. However, the distinct mechanisms, in particular, the role of oxidative stress on the consequences of coronavirus infection, have not been well investigated, which is the purpose of the present study. The standard semen parameters, its pro- and antioxidant system state, as well as the level of sperm DNA fragmentation, were assessed in 17 semen samples of men five months after the coronavirus infection and in 22 age-matched control patients. We determined that the DNA fragmentation rate negatively correlated with the period after coronavirus recovery, as well as seminal fluid superoxide dismutase activity and uric acid level. It was demonstrated that COVID-19 is not always associated with increased DNA fragmentation, allowing them to be considered as two independent factors. Thus, the most significant changes were noted in the samples of men after COVID-19 and abnormal TUNEL results: increased round cell number, decreased seminal fluid's nitrotyrosine level, and total antioxidant capacity and Zn, as well as an increased 8-hydroxy-2'-deoxyguanosine level within spermatozoa. The data obtained indicate that increased DNA fragmentation and diminished semen quality in men can be the result of an imbalance in semen pro- and antioxidant components after COVID-19.

Shcherbitskaia A.D. , Komarova E.M. , Milyutina Y.P. , Ishchuk M.A. , Sagurova Y.M. , Safaryan G.K. , Lesik E.A. , Gzgzyan A.M. , Bespalova O.N. , Kogan I.Y.
Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19
International Journal of Molecular Sciences. 2022. V.23. N17. 10060 . DOI: 10.3390/ijms231710060 WOS OpenAlex

Submitted:	Jul 1, 2022
Accepted:	Aug 30, 2022
Published online:	Sep 2, 2022

Web of science:	WOS:000851971800001
OpenAlex:	W4294741524

DB	Citing
Web of science	18
OpenAlex	23