High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression

High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression Научная публикация

Журнал

Life
ISSN: 2075-1729

Вых. Данные

Год: 2021, Том: 11, Номер: 10, Номер статьи : 1055, Страниц : DOI: 10.3390/life11101055

Авторы

Vashukova Elena S. ¹ , Kozyulina Polina Y. ^1,2 , Illarionov Roman A. ^1,3,4 , Yurkina Natalya O. ¹ , Pachuliia Olga V. ¹ , Butenko Mariya G. ¹ , Postnikova Tatyana B. ^1,5 , Ivanova Lada A. ⁵ , Eremeeva Dina R. ⁶ , Zainulina Marina S. ⁶ , Bespalova Olesya N. ¹ , Glotov Andrey S. ^1,5

Организации

1	Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia.
2	Signal Regulation Laboratory, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia
3	Department of Chemical and Biotechnology, St. Petersburg State Institute of Technology, Technical University, 190013 St. Petersburg, Russia
4	Institute of Translational Biomedicine, St. Petersburg State University, 199034 St. Petersburg, Russia
5	Antenatal Clinic No.26 Maternity Hospital No.10, 198259 St. Petersburg, Russia
6	II Obstetric Department Pathology of Pregnancy, V. F. Snegirev Maternity Hospital No.6, 192014 St. Petersburg, Russia

Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy (p-value adjusted <0.05). In addition, we observed differences for 36 miRNAs between plasma and serum (p-value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR (p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities.

Vashukova E.S. , Kozyulina P.Y. , Illarionov R.A. , Yurkina N.O. , Pachuliia O.V. , Butenko M.G. , Postnikova T.B. , Ivanova L.A. , Eremeeva D.R. , Zainulina M.S. , Bespalova O.N. , Glotov A.S.
High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
Life. 2021. V.11. N10. 1055 . DOI: 10.3390/life11101055 WOS Scopus РИНЦ OpenAlex

Опубликована online:

8 окт. 2021 г.

Web of science:	WOS:000711395100001
Scopus:	eid_2-s2.0-85117339252
РИНЦ:	47514821
OpenAlex:	W3203575840

БД	Цитирований
OpenAlex	18
Web of science	12