Chromosome hydroxymethylation patterns in human zygotes and cleavage-stage embryos

Chromosome hydroxymethylation patterns in human zygotes and cleavage-stage embryos Научная публикация

Журнал

Reproduction
ISSN: 1470-1626

Вых. Данные

Год: 2015, Том: 149, Номер: 3, Страницы: 223-233 Страниц : 11 DOI: 10.1530/rep-14-0343

Авторы

Efimova Olga A ^1,2 , Pendina Anna A ^1,2,3 , Tikhonov Andrei V ^1,2,3 , Fedorova Irina D ¹ , Krapivin Mikhail I ² , Chiryaeva Olga G ^1,4,5 , Shilnikova Evgeniia M ^1,2 , Bogdanova Mariia A ^1,6 , Kogan Igor Yu ^1,7 , Kuznetzova Tatyana V ^1,2 , Gzgzyan Alexander M ^1,2 , Ailamazyan Edward K ^1,2,7 , Baranov Vladislav S ^1,2,5

Организации

1	D.O. Ott Research Institute of Obstetrics and Gynecology, Mendeleevskaya line, 3, 199034 St Petersburg, Russia,
2	St Petersburg State University, Universitetskaya nab.7/9, 199034 St Petersburg, Russia,
3	Center for Medical Genetics, Tobolskaya ul., 5, 194044 St Petersburg, Russia,
4	St Petersburg State Pediatric Medical University, Litovskaya ul., 2, 194100 St Petersburg, Russia,
5	S.M. Kirov Military Medical Academy, Lebedeva ul., 6, 194044 St Petersburg, Russia,
6	N.I. Pirogov National Medical-Surgery Center, St Petersburg Clinic Complex, nab. Fontanki, 154, 190103 St Petersburg, Russia and
7	I.P. Pavlov First St Petersburg State Medical University, L'va Tolstogo ul., 6/8, 197022 St Petersburg, Russia

We report the sequential changes in 5-hydroxymethylcytosine (5hmC) patterns in the genome of human preimplantation embryos during DNA methylation reprogramming. We have studied chromosome hydroxymethylation and methylation patterns in triploid zygotes and blastomeres of cleavage-stage embryos. Using indirect immunofluorescence, we have analyzed the localization of 5hmC and its co-distribution with 5-methylcytosine (5mC) on the QFH-banded metaphase chromosomes. In zygotes, 5hmC accumulates in both parental chromosome sets, but hydroxymethylation is more intensive in the poorly methylated paternal set. In the maternal set, chromosomes are highly methylated, but contain little 5hmC. Hydroxymethylation is highly region specific in both parental chromosome sets: hydroxymethylated loci correspond to R-bands, but not G-bands, and have well-defined borders, which coincide with the R/G-band boundaries. The centromeric regions and heterochromatin at 1q12, 9q12, 16q11.2, and Yq12 contain little 5mC and no 5hmC. We hypothesize that 5hmC may mark structural/functional genome 'units' corresponding to chromosome bands in the newly formed zygotic genome. In addition, we suggest that the hydroxymethylation of R-bands in zygotes can be treated as a new characteristic distinguishing them from G-bands. At cleavages, chromosomes with asymmetrical hydroxymethylation of sister chromatids appear. They decrease in number during cleavages, whereas totally non-hydroxymethylated chromosomes become numerous. Taken together, our findings suggest that, in the zygotic genome, 5hmC is distributed selectively and its pattern is determined by both parental origin of chromosomes and type of chromosome bands - R, G, or C. At cleavages, chromosome hydroxymethylation pattern is dynamically changed due to passive and non-selective overall loss of 5hmC, which coincides with that of 5mC.

Efimova O.A. , Pendina A.A. , Tikhonov A.V. , Fedorova I.D. , Krapivin M.I. , Chiryaeva O.G. , Shilnikova E.M. , Bogdanova M.A. , Kogan I.Y. , Kuznetzova T.V. , Gzgzyan A.M. , Ailamazyan E.K. , Baranov V.S.
Chromosome hydroxymethylation patterns in human zygotes and cleavage-stage embryos
Reproduction. 2015. V.149. N3. P.223-233. DOI: 10.1530/rep-14-0343 OpenAlex

Поступила в редакцию:	6 июл. 2014 г.
Принята к публикации:	11 дек. 2014 г.

OpenAlex:

W2159377799

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