Study of acetylated histone h3k9 – an active chromatin mark – in chromosomes from adult and fetal human lymphocytes

Study of acetylated histone h3k9 – an active chromatin mark – in chromosomes from adult and fetal human lymphocytes Full article

Journal

Экологическая генетика
ISSN: 1811-0932 , E-ISSN: 2411-9202

Output data

Year: 2019, Volume: 17, Number: 3, Pages: 111-117 Pages count : 7 DOI: 10.17816/ecogen173111-117

Authors

Efimova Olga A. ¹ , Pendina Anna A. ^1,2 , Lezhnina Yuliia G. ² , Tikhonov Andrei V. ^1,2 , Chiryaeva Olga G. ¹ , Petrova Lyubov I. ¹ , Dudkina Vera S. ¹ , Koltsova Alla S. ^1,3 , Krapivin Mikhail I. ^1,3 , Petrovskaia-Kaminskaia Anastasiia V. ^1,3 , Talantova Olga E. ¹ , Kuznetzova Tatiana V. ¹ , Baranov Vladislav S. ^1,3

Affiliations

1	D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
2	Сenter for Medical Genetics
3	Saint Petersburg State University

Background. Incorrect epigenetic modifications of the human genome may result in epigenetic disorders, thus, highlighting the necessity of studying chromosome epigenetic patterns in human development. Aim of the study: a comparative analysis of acetylated histone H3K9 (AcH3K9) patterns in human metaphase chromosomes from the lymphocytes of adults and fetuses. Materials and methods. The immunocytochemical detection of AcH3K9 in the metaphase chromosomes from PHA-stimulated peripheral lymphocytes of 13 adults and cord blood lymphocytes of 10 fetuses at 20-22 weeks of gestation. Results. Both in the chromosomes of the adults and the fetuses, AcH3K9 accumulated in the R- and T-, but not G-bands and avoided the regions of pericentromeric heterochromatin of the chromosomes 1, 9 and 16. When comparing the adult and the fetal chromosomes, different levels of AcH3K9 were revealed in a few bands: 2q31, 5p13, 5p15 and 16p13 had higher level of Н3К9 acetylation in adults, in contrast to 9q13 which was hyperacetylated in fetuses. Conclusion. The АсН3К9 distribution in metaphase chromosomes is band-specific and is similar between the adults and the fetuses, excluding a few bands with different acetylation levels.

Efimova O.A. , Pendina A.A. , Lezhnina Y.G. , Tikhonov A.V. , Chiryaeva O.G. , Petrova L.I. , Dudkina V.S. , Koltsova A.S. , Krapivin M.I. , Petrovskaia-Kaminskaia A.V. , Talantova O.E. , Kuznetzova T.V. , Baranov V.S.
Study of acetylated histone h3k9 – an active chromatin mark – in chromosomes from adult and fetal human lymphocytes
Экологическая генетика. 2019. V.17. N3. P.111-117. DOI: 10.17816/ecogen173111-117 OpenAlex

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