A New Relevant Cellular Model of Uterine Leiomyoma: Method of Isolation and Prospects of Use

A New Relevant Cellular Model of Uterine Leiomyoma: Method of Isolation and Prospects of Use Научная публикация

Журнал

Applied Biochemistry and Microbiology
ISSN: 1608-3024

Вых. Данные

Год: 2025, Том: 61, Номер: 9, Страницы: 1734–1742 Страниц : DOI: 10.1134/S0003683825700553

Ключевые слова

uterine leiomyoma, cell model, MED12, HMGA2

Авторы

Shved N.Yu. ¹ , Egorova A.A. ¹ , Shtykalova S.V. ¹ , Osinovskaya N.S. ¹ , Kogan I.Yu. ¹ , Kiselev A.V. ¹

Организации

1	Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, 199034 Russia

Информация о финансировании (1)

Министерство науки и высшего образования Российской Федерации

FGWN-2022-0004

Uterine leiomyoma (UL) is a benign tumor of monoclonal origin developing from smooth muscle cells and containing varying amounts of fibrous connective tissue. Leiomyomas and myometrium are similar in cellular composition but differ in the cellular transcriptome profiles. A rapid and reliable method for isolating and culturing primary UL cells is described in this paper. Conditions for growing and maintaining a monolayer culture of UL cells in vitro were optimized. The effect of sex steroid hormones, estradiol and progesterone, on UL genetic markers was evaluated. To prevent the loss of driver mutations in the MED12 and HMGA2 genes, the use of the UL cell model cultured in a specialized nutrient medium with the addition of progesterone at the first passage was proposed.

Shved N.Y. , Egorova A.A. , Shtykalova S.V. , Osinovskaya N.S. , Kogan I.Y. , Kiselev A.V.
A New Relevant Cellular Model of Uterine Leiomyoma: Method of Isolation and Prospects of Use
Applied Biochemistry and Microbiology. 2025. V.61. N9. P.1734–1742. DOI: 10.1134/S0003683825700553 WOS Scopus OpenAlex

Швед Н.Ю. , Егорова А.А. , Штыкалова С.В. , Осиновская Н.С. , Коган И.Ю. , Киселев А.В.
Новая релевантная клеточная модель лейомиомы матки: способ получения и перспективы использования
Биотехнология. 2024. Т.40. №4. С.63-72. DOI: 10.56304/s0234275824040094 OpenAlex

Поступила в редакцию:	28 мая 2024 г.
Принята к публикации:	26 июл. 2024 г.
Опубликована в печати:	16 янв. 2026 г.
Опубликована online:	16 янв. 2026 г.

≡ Web of science:	WOS:001663662800008
≡ Scopus:	2-s2.0-105027650256
≡ OpenAlex:	W7124469523